Restriction enzymes (or restriction endonucleases) are proteins that cut DNA at specific recognition sequences. They are essential tools in molecular biology for DNA manipulation and cloning.

• Recognition sequence: Specific DNA sequence recognized by the enzyme
• Cut position: Where the enzyme cuts relative to the recognition sequence
• Overhang: Type of ends created after cutting (5', 3', or blunt)
• Temperature: Optimal temperature for enzyme activity
• Buffer conditions: Required salt and pH conditions

1. Enter your DNA sequence
2. Select restriction enzymes to analyze
3. Click calculate to find:
• Cut site positions
• Fragment sizes
• Overhang types
• Reaction conditions

Note: For best results:
• Use clean DNA sequence (A, T, C, G only)
• Consider enzyme compatibility for multiple digests
• Check buffer and temperature requirements

• Cut Positions: Numbers indicate base positions where cuts occur
• Fragments: DNA pieces produced after digestion
• Overhangs: Sticky ends created at cut sites
• Compatibility: Whether selected enzymes can work together

• Use high-quality DNA
• Ensure complete digestion
• Consider double digests carefully
• Include positive controls
• Check enzyme storage conditions
• Verify fragment sizes by gel electrophoresis