Cell Culture Dilution Calculator

Cell culture dilution is a fundamental technique in cell biology and microbiology where cells in culture are diluted to achieve a specific concentration. This process is crucial for maintaining healthy cell growth, performing experiments, and preparing cells for various applications.

Accurate cell culture dilutions are essential for: • Maintaining optimal cell density for growth • Setting up experiments with specific cell numbers • Preparing cells for assays or cell counting • Standardizing protocols across different experiments • Ensuring reproducibility in research

1. Enter your initial cell concentration (cells/mL) 2. Input your desired target concentration 3. Specify the final volume needed 4. Click calculate to determine: • Volume of cell stock solution needed • Volume of medium to add • The resulting dilution factor

The calculator provides: • Stock Volume: Amount of your original cell suspension to use • Medium Volume: Amount of fresh medium to add • Dilution Factor: The fold-dilution of your original sample These calculations ensure precise and reproducible cell culture work.

• Always use sterile technique • Mix cell suspensions gently but thoroughly • Use calibrated pipettes • Account for dead space in containers • Consider cell settling time • Document all dilution steps